THIS SITE IS BEST VIEWED IN GOOGLE CHROME....................................................NOTE: Copyright Issues - Any image on this blog showing Blog's name or Blog address is not claimed to be created by the Blog's author unless otherwise specified.These images have been acquired from the web and Bha Bha Blogsghip bears no copyrights of these images.If you have any objection over any image or content please e-mail to bharatrbh@yahoo.com.

Sunday, November 6, 2011

USP Classification of HPLC Columns


All HPLC packings are not alike, and no single column can perform a various of desired separations. HPLC packings differ in hydrophobicity, surface coverage, surface area, pore size and particle shape.


Classification of HPLC Columns as per United State pharmacopoeia.

L1
Octadecyl silane chemically bonded to porous silica or ceramic microparticles, 1.5 to 10 μm in diameter, or a monolithic rod.
L2
Octadecyl silane chemically bonded to silica gel of a controlled surface
porosity that has been bonded to a solid spherical core, 30 to 50 μm in diameter
L3
Porous silica particles, 5 to 10 μm in diameter.
L4
Silica gel of controlled surface porosity bonded to a solid spherical core,
30 to 50 μm in diameter
L5
Alumina of controlled surface porosity bonded to a solid spherical core,
30 to 50 μm in diameter.
L6
Strong cation-exchange packing: sulfonated fluorocarbon polymer coated
on a solid spherical core, 30 to 50 μm in diameter.
L7
Octyl silane chemically bonded to totally porous silica particles, 1.5 to 10 μm in diameter.
L8
An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally porous silica gel support, 3 to 10 μm in diameter
L9
Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter
L10
Nitrile groups chemically bonded to porous silica particles, 3 to 10 μm in diameter
L11
Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter.
L12
Strong anion-exchange packing made by chemically bonding a quaternary
amine to a solid silica spherical core, 30 to 50 μm in diameter
L13
Trimethylsilane chemically bonded to porous silica particles, 3 to 10 μm in diameter
L14
Silica gel having a chemically bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10 µm in diameter.
L15
Hexyl silane chemically bonded to totally porous silica particles, 3 to 10 μm in diameter.
L16
Dimethyl silane chemically bonded to totally porous silica particles, 5 to 10 μm in diameter
L17
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 μm in diameter
L18
Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 μm in diameter
L19
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 9 μm in diameter
L20
Dihydroxypropane groups chemically bonded to porous silica particles,5 to 10 μm in diamete
L21
A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 μm in diameter.
L22
A cation exchange resin made of porous polystyrene gel with sulfonic acid groups, about 10 μm in size
L23
An anion exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 10 μm in size.
L24
A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl
groups on the matrix surface, 32 to 63 μm in diameter
L25
Packing having the capacity to separate compounds with a MW range from 100 to 5000 daltons (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base,
crosslinked with poly-hydroxylated ether (surface contained some residual
carboxyl functional groups) was found suitable.
L26
Butyl silane chemically bonded to totally porous silica particles, 5 to 10 μm in diameter
L27
Porous silica particles, 30 to 50 μm in diameter
L28
A multifunctional support, which consists of a high purity, 100 Å, spherical
silica substrate that has been bonded with anionic (amine) functionality
in addition to a conventional reversed phase C8 functionality.
L29
Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based
polybutadiene spherical particles, 5 μm diameter with a pore diameter of 80 Å.
L30
Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10 μm in diameter
L31
A strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5 μm macroporous particles having a pore size of 2000 Å
and consisting of ethylvinylbenzene cross-linked with 55 % divinyl benzene.
L32
A chiral ligand-exchange packing- L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10 μm in diameter
L33
Packing having the capacity to separate proteins of 4,000 to 400,000 daltons. It is spherical, silica-based and processed to provide pH stability.
L34
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinyl benzene copolymer in the lead form, about 9 μm in diameter
L35
A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150 Å.
L36
3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 μm aminopropyl silica
L37
Polymethacrylate gel packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000D.
L38
Methacrylate-based size-exclusion packing for water-soluble samples.
L39
Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin
L40
Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles,
5 μm to 20 μm in diameter.
L41
Immobilized α-acid glycoprotein on spherical silica particles, 5 μm in diameter.
L42
Octylsilane and octadecylsilane groups chemically bonded to porous silica particles,
5 μm in diameter
L43
Pentafluorophenyl groups chemically bonded to silica particles, 5 to 10 μm in diameter
L44
A multifunctional support, which consists of a high purity, 60 Å, spherical silica substrate
that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to
a conventional reversed phase C8 functionality.
L45
Beta cyclodextrin bonded to porous silica particles, 5 to 10 μm in diameter
L46
Polystyrene/divinylbenzene substrate agglomerated with quaternary amine
functionalized latex beads, 10 μm in diameter
L47
High capacity anion-exchange microporous substrate, fully functionalized with a
trimethylamine group, 8 μm in diameter.
L48
Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous,
anion-exchange microbeads, 15 μm in diameter
L49
A reversed-phase packing made by coating a thin layer of polybutadiene on
to spherical porous zirconia particles, 3 to 10 μm in diameter
L50
Multifunction resin with reversed-phase retention and strong anion-exchange
functionalities. The resin consists of ethylvinylbenzene, 55 % cross-linked with
divinylbenzene copolymer, 3 to 15 μm in diameter, and a surface area of not less
than 350 m2/g, substrate is coated with quaternary ammonium functionalized
latex particles consisting of styrene cross-linked with divinylbenzene.
L51
Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles,
5 to 10 μm in diameter.
L52
A strong cation exchange resin made of porous silica with sulfopropyl groups, 5 to 10 μm in diameter.
L53
Weak cation-exchange resin consisting of ethylvinylbenzene, 55 % cross-linked with
divinylbenzene copolymer, 3 to 15 μm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 µm in diameter
L54
A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about 13 µm in diameter
L55
A strong cation exchange resin made of porous silica coated with polybutadiene-maleic
acid copolymer, about 5 µm in diameter.
L56
Isopropyl silane chemically bonded to totally porous silica particles, 3 to 10 μm in diameter.
L57
A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 μm in diameter, with a pore size of 120 angstroms.
L58
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 7 to 11 μm diameter
L59
To separate proteins by molecular weight over the range of 10 to 500 kDa. Spherical 10 μm, silica-based, and processed to provide hydrophilic characteristics
and pH stability
L60
Spherical, porous silica gel, 3 to 10 μm in diameter, surface has been covalently modified with palmitamidopropyl groups and endcapped.
L61
Hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked
core of 13 μm microporous particles, pore size less than 10 Å, and consisting of
ethylvinylbenzene cross-linked with 55 % divinylbenzene with a latex coating
composed of 85 nm diameter microbeads bonded with alkanol quarternary
ammonium ions (6 %).
L62
C30 silane bonded phase on a fully porous spherical silica, 3 to 15 μm in diameter

No comments:

Post a Comment

Feel Free to Comment

Top 5 Popular Posts